Copper removal efficacy and stress tolerance potential of Leptolyngbya sp. GUEco1015

Abstract

Cyanobacteria, a group of microalgae are the potent organism having the ability to survive in the copper rich environment and recently gained too much attention for their profuse proliferation in such water bodies. Amongst the members of cyanobacteria, the current study was conducted on Leptolyngbya sp. GUEco1015, collected from hydrocarbon rich water bodies of Assam, India. Morphological images of treated samples showed a remarkable damage in the cell surface as well as the organelles over the control. Biochemical results revealed a significant increase of enzymatic and non-enzymatic antioxidants during oxidative damage of Cu2+. But, ascorbate in 1.2 ppm (p < 0.01), 1.5 ppm (p < 0.001) and catalase content 1.5 ppm (p < 0.05) showed a significant reduction after a certain level. The cells were optimized to evaluate the maximum Cu2+ removal potential by the cells related to growth. Initial metal concentration 0.1 ppm, pH 7.5, temperature 25 °C and shaking rate 100 rpm are the optimized abiotic parameters which showed maximum 83% of Cu2+ removal. FTIR spectroscopy and EDX data has identified a number of notable functional groups that were involved in Cu2+ binding mechanism and revealed a distinctive peak of Cu with 0.41 wt % which makes the species as one of the competent copper adsorbents.

Keywords

• Cyanobacterium
• Leptolyngbya sp. GUEco 1015
• Copper
• Antioxidants
• Removal efficacy