Nature Communications (Jul 2017)

Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

  • Luca Lanzanò,
  • Lorenzo Scipioni,
  • Melody Di Bona,
  • Paolo Bianchini,
  • Ranieri Bizzarri,
  • Francesco Cardarelli,
  • Alberto Diaspro,
  • Giuseppe Vicidomini

DOI
https://doi.org/10.1038/s41467-017-00117-2
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 10

Abstract

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The measurement of molecular diffusion at sub-diffraction scales has been achieved in 2D space using STED-FCS, but an implementation for 3D diffusion is lacking. Here the authors present an analytical approach to probe diffusion in 3D space using STED-FCS and measure the diffusion of EGFP at different spatial scales.