Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2

Jinxin Xu; Xin Jiang; Yulong Zhang; Yan Dong; Changli Ma; Hanqiu Jiang; Taisen Zuo; Rui Chen; Yubin Ke; He Cheng; Howard Wang; Jinsong Liu

doi:10.1038/s42003-022-03892-x

Communications Biology (Sep 2022)

Multiscale characterization reveals oligomerization dependent phase separation of primer-independent RNA polymerase nsp8 from SARS-CoV-2

Jinxin Xu,
Xin Jiang,
Yulong Zhang,
Yan Dong,
Changli Ma,
Hanqiu Jiang,
Taisen Zuo,
Rui Chen,
Yubin Ke,
He Cheng,
Howard Wang,
Jinsong Liu

Affiliations

Jinxin Xu: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Xin Jiang: Neutron Science Platform, Songshan Lake Materials Laboratory
Yulong Zhang: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Yan Dong: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Changli Ma: Spallation Neutron Source Science Center
Hanqiu Jiang: Spallation Neutron Source Science Center
Taisen Zuo: Spallation Neutron Source Science Center
Rui Chen: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Yubin Ke: Spallation Neutron Source Science Center
He Cheng: Spallation Neutron Source Science Center
Howard Wang: Neutron Science Platform, Songshan Lake Materials Laboratory
Jinsong Liu: State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s42003-022-03892-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 9

Abstract

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The phase separation behaviour of non-structure protein nsp8 of SARS CoV2 in the primer synthesis mechanism is presented, underpinning the replication of coronavirus.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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