Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections
Vinicius de Queiroz Albuquerque,
Maria Janeila Carvalho Soares,
Maria Nágila Carneiro Matos,
Rafaela Mesquita Bastos Cavalcante,
Jesús Alberto Pérez Guerrero,
Tigressa Helena Soares Rodrigues,
Geovany Amorim Gomes,
Rodrigo Fonseca de Medeiros Guedes,
Débora de Souza Collares Maia Castelo-Branco,
Isaac Neto Goes da Silva,
Victor Alves Carneiro
Affiliations
Vinicius de Queiroz Albuquerque
Department of Veterinary Sciences, State University of Ceará—Itaperi Campus, Fortaleza 60714-903, Brazil
Maria Janeila Carvalho Soares
Center for Bioprospecting and Applied Molecular Experimentation (NUBEM), University Center INTA-UNINTA, Sobral 62050-100, Brazil
Maria Nágila Carneiro Matos
Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, Brazil
Rafaela Mesquita Bastos Cavalcante
Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, Brazil
Jesús Alberto Pérez Guerrero
Laboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, Brazil
Tigressa Helena Soares Rodrigues
Center of Exact Science and Technology, State University of Acaraú Valley, Sobral 62040-370, Brazil
Geovany Amorim Gomes
Center of Exact Science and Technology, State University of Acaraú Valley, Sobral 62040-370, Brazil
Rodrigo Fonseca de Medeiros Guedes
Group of Applied Medical Microbiology, Microbiology Department, Federal University of Ceará, Fortaleza 60430-160, Brazil
Débora de Souza Collares Maia Castelo-Branco
Group of Applied Medical Microbiology, Microbiology Department, Federal University of Ceará, Fortaleza 60430-160, Brazil
Isaac Neto Goes da Silva
Department of Veterinary Sciences, State University of Ceará—Itaperi Campus, Fortaleza 60714-903, Brazil
Victor Alves Carneiro
Center for Bioprospecting and Applied Molecular Experimentation (NUBEM), University Center INTA-UNINTA, Sobral 62050-100, Brazil
The aim of this study was to evaluate the phytochemical profile of Cinnamomum zeylanicum essential oil (CZEO) and their antimicrobial and antibiofilm activity against Staphylococcus strains isolated from canine otitis. First, the CZEO chemical composition was determined by gas chromatography-mass spectrometry (CG-MS). External otitis samples collected from dogs were submitted to staphylococcal isolation, followed by MALDI-TOF mass spectrometry identification. The antimicrobial action was tested against the isolates using the disk-diffusion and microdilution methods. The antibiofilm activity was evaluated by CZEO-based concentrations, subMIC for biofilm formation and supraMIC against preformed biofilm, quantified by crystal violet (CV) staining and CFU counting. The chemical analysis revealed that (E)-cinnamaldehyde, eugenol and (E)-cinnamyl acetate were the main compounds in the CZEO, representing 77.42, 8.17 and 4.50%, respectively. Two strains of three different species, S. saprophyticus, S. schleiferi and S. pseudintermedius, were identified. The disk-diffusion test showed an inhibitory zone diameter, ranging from 34.0 to 49.5 mm, while the MIC and MBC values were around 500 and 1000 µg/mL. SubMIC demonstrated an inhibition on biofilm formation against 4 out the 6 strains tested. On mature biofilm, the CZEO-based supraMIC groups had slightly change on biomass, however, the biofilm cell viability decreased the CFU in 3 magnitude orders.
