The Effectiveness Of Tofu Liquid Waste As A Natural Phytoestrogen For Mandibular Bone Of Ovariectomized Rats

Abstract

Estrogen is a steroid compound that has many benefits for the body's physiology. Hypoestrogenic women could have periodontitis in their mouth. Hormone replacement therapy (HRT) is a therapy that used to reduce the disturbance caused by estrogen deficiency. However, HRT has many side effects. Theoretical study shows that isoflavones in tofu liquid waste has an element of a steroid compound. The purpose of this study was to prove the effectiveness of isoflavones in the tofu liquid waste by examining the microscopic structure, estrogen receptor expression and MDA levels in rat mandibular bone post-ovariectomy. This research method use a true experimental laboratory with randomized post test control group design. Twenty-four female wistar rats were divided randomly into six groups, consisted of a group of normal rat that does’nt ovariectomized (C1), 4 weeks ovariectomized rats (C2), 8 weeks ovariectomized rats (C3) but no tofu liquid waste was given, and 8 weeks ovariectomized rats that given tofu liquid waste at the end of the 4th week of ovariectomy with three different doses (C4 = 1.2, C5 = 6; C6 = 12 ml / kg). Histopathological slide used to see the changes resulted in a number of osteoclasts, osteocytes, periodontal ligament width, alveolar bone height, the amount of estrogen receptor expression and MDA levels. The results of statistical tests show that there is a difference in the rats between six different groups (ANOVA, p 0.05) and there is a close relationship between the dose of the tofu liquid waste with all parameter. The conclusion of this study is tofu liquid waste could improve the microscopic structure, estrogen receptor expression and MDA levels in rat mandibular bone post-ovariectomy.

Keywords

• menopause
• periodontitis
• isoflavones
• tofu liquid waste