The Characterization of Haemaglutinin Protein of Vili Vibrio alginolyticus as Adhesion Molecule on Epithelial Cells Receptor of Humpback Grouper (Cromileptes altivelis)

Abstract

Vibriosis was a crucial problem and a primer infection causing mortality of humpback grouper. Virulence factor of vibrio, such as vili, is an adhesion molecule protein based on haemaglutination test to blood serum of humpback grouper. This research was aimed to characterize an adhesion molecule of vili protein of V. alginolyticus on epithelial cells of Humpback grouper as haemaglutinin protein. The methods are laboratory exploration by isolate epithelial cells of Humpback grouper, characterize a receptor and test vili protein to adhesion molecule that is epithelial cell of Humpback grouper. The result of protein haemaglutinin test to vili V. alginolyticus show that an adhesion molecule with a molecule weight of 38.98 kDa is an adhesin vili V. alginolyticus and a haemaglutinin. The adhesion pattern of V. alginolyticus on epithelial cells of Humpback grouper exposed to protein haemaglutinin of vili show both diffuse and localize pattern. Research conclusion was the haemaglutinin protein of vili V. alginolyticus was an adhesin having a role to both virulence process of bacteria and attachment process to receptor of epithelial cells of Humpback grouper. Keywords: adhesion molecule, epithelial cell, vili, V. alginolyticus, C. altivelis