Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1

Bumpei Samata; Daisuke Doi; Kaneyasu Nishimura; Tetsuhiro Kikuchi; Akira Watanabe; Yoshimasa Sakamoto; Jungo Kakuta; Yuichi Ono; Jun Takahashi

doi:10.1038/ncomms13097

Nature Communications (Oct 2016)

Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1

Bumpei Samata,
Daisuke Doi,
Kaneyasu Nishimura,
Tetsuhiro Kikuchi,
Akira Watanabe,
Yoshimasa Sakamoto,
Jungo Kakuta,
Yuichi Ono,
Jun Takahashi

Affiliations

Bumpei Samata: Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
Daisuke Doi: Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
Kaneyasu Nishimura: Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
Tetsuhiro Kikuchi: Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
Akira Watanabe: Department of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University
Yoshimasa Sakamoto: Group for Antibody Engineering, KAN Research Institute Inc
Jungo Kakuta: Group for Seed Biologics, KAN Research Institute Inc.
Yuichi Ono: Group for Neuronal Differentiation and Development, KAN Research Institute Inc.
Jun Takahashi: Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University

DOI: https://doi.org/10.1038/ncomms13097
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 11

Abstract

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Midbrain dopaminergic neurons generated from stem cells show promise for the treatment of Parkinson’s disease. Here, the authors use the cell surface marker, LRTM1, to enrich the midbrain dopaminergic progenitors and show improved motor function/cell survival when grafted into rat/monkey brains, respectively.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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