Metabolomic Profiling of Mango (<i>Mangifera indica</i> Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Abstract

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p −5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p 2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p p 2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

Keywords

• <i>Mangifera indica</i>
• oxidative stress
• relaxation
• rat ileum
• high-resolution orbitrap mass spectrometry
• metabolome