Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat

Abstract

ABSTRACT: Enrofloxacin, a veterinary antibiotic that persists in food, poses a risk to human health. Here, a monoclonal antibody against enrofloxacin, 1H12, was prepared based on the hapten ENR-1, and showed excellent sensitivity with a 50% inhibitory concentration (IC50) of 0.03 ng/mL. Using this antibody, 2 lateral-flow immunochromatographic assays were developed for determination of enrofloxacin in egg, milk, honey, and chicken meat samples. The detection ranges (IC20–IC80) were 0.16–0.82 ng/g, 0.24–1.8 ng/g, 0.25–3.6 ng/g, and 0.61–3.9 ng/g by colloidal gold-immunochromatographic sensor (CG-ICS) analysis, and 0.022–0.42 ng/g, 0.054–0.42 ng/g, 0.069–1.4 ng/g, and 0.19–2.2 ng/g by Eu-fluorescence-immunochromatographic sensor (EF-ICS) analysis. The intraassay and interassay recovery rates were 88.9 to 108.5% with coefficients of variation of 1.3 to 7.0% by CG-ICS analysis, and 88.6 to 113.6% with coefficients of variation of 1.3 to 8.1% by EF-ICS analysis. Thus, our newly developed ICS are sensitive and reliable, providing an option for rapid quantitative detection of enrofloxacin in food samples.

Keywords

• antibody
• antibiotic
• enrofloxacin
• immunochromatographic
• strip