Nuclei multiplexing with barcoded antibodies for single-nucleus genomics

Jellert T. Gaublomme; Bo Li; Cristin McCabe; Abigail Knecht; Yiming Yang; Eugene Drokhlyansky; Nicholas Van Wittenberghe; Julia Waldman; Danielle Dionne; Lan Nguyen; Philip L. De Jager; Bertrand Yeung; Xinfang Zhao; Naomi Habib; Orit Rozenblatt-Rosen; Aviv Regev

doi:10.1038/s41467-019-10756-2

Nature Communications (Jul 2019)

Nuclei multiplexing with barcoded antibodies for single-nucleus genomics

Jellert T. Gaublomme,
Bo Li,
Cristin McCabe,
Abigail Knecht,
Yiming Yang,
Eugene Drokhlyansky,
Nicholas Van Wittenberghe,
Julia Waldman,
Danielle Dionne,
Lan Nguyen,
Philip L. De Jager,
Bertrand Yeung,
Xinfang Zhao,
Naomi Habib,
Orit Rozenblatt-Rosen,
Aviv Regev

Affiliations

Jellert T. Gaublomme: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Bo Li: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Cristin McCabe: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Abigail Knecht: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Yiming Yang: Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology Massachusetts General Hospital and Harvard Medical School
Eugene Drokhlyansky: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Nicholas Van Wittenberghe: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Julia Waldman: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Danielle Dionne: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Lan Nguyen: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Philip L. De Jager: Center for Translational & Computational Neuroimmunology, Columbia University Medical Center
Bertrand Yeung: BioLegend Inc.
Xinfang Zhao: BioLegend Inc.
Naomi Habib: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Orit Rozenblatt-Rosen: Klarman Cell Observatory, Broad Institute of Harvard and MIT
Aviv Regev: Klarman Cell Observatory, Broad Institute of Harvard and MIT

DOI: https://doi.org/10.1038/s41467-019-10756-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 8

Abstract

Read online

Single-nucleus RNA-seq enables interrogation of complex tissues but is limited due to batch effects and processing costs. Here the authors use barcoded antibodies against the nuclear pore complex to label nuclei from distinct samples, and develop a computational tool to assign the sample of origin.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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