Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs
Shun Cheng,
Man Liu,
Yong-chun Wei,
Mei-li Chi,
Jian-bo Zheng,
Wen-ping Jiang,
Shi-li Liu,
Xiao-ying Hang,
Miao Peng,
Fei Li,
Dan-li Wang
Affiliations
Shun Cheng
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China
Man Liu
Food and Drug Inspection and Testing Center of Deqing, Huzhou Zhejiang 313200 China
Yong-chun Wei
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Mei-li Chi
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Jian-bo Zheng
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Wen-ping Jiang
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Shi-li Liu
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Xiao-ying Hang
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Miao Peng
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China
Fei Li
Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China; Corresponding author.
Dan-li Wang
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China; Corresponding author.
We explored the effects of different conditions on the artificial incubation of redclaw crayfish eggs in an effort to improve this process. Samples at the egg and juvenile stages were selected. The samples at different stages were separated from the pleopods, then they were placed in incubator boxes and sterilized with different disinfectant solutions. The density was 300,400 and 500 eggs/incubator box, the vibration frequency was 11,16 and 26 vibrations/min, and the water circulation cycle was 2.1, 4.8 and 7.1 cycles/h. The results showed the eggs disinfected with 3000 ppm formaldehyde for 15 min had stronger antioxidant capacity. The hatching and survival rates of five pairs of appendage stage group were significantly lower than those of other groups. In the egg stage, acid phosphatase (ACP) level of compound eye pigmentation stage group was significantly higher than those of other groups. In the juvenile stage, malondialdehyde (MDA) content of five pairs of appendage stage group was significantly higher than those of other groups. The survival rate of 500 eggs/box group was significantly higher than that of other groups. In the egg stage, alkaline phosphatase (AKP) level of 400 eggs/box group was significantly higher than that of other groups. The survival rate of 11 vibrations/min group was significantly higher than that of other groups. In the egg stage, ACP and AKP levels of 11 vibrations/min group were significantly higher than those of 26 vibrations/min group. In the juvenile stage, superoxide dismutase (SOD), ACP and AKP levels of 11 vibrations/min group was significantly higher than those of 26 vibrations/min group. In the juvenile stage, AKP level of 4.8 cycles/h group was significantly lower than that of other groups. In conclusion, egg development at the stage after seven pairs of appendages, with a density of 400 eggs/box, vibration frequencies set at 11 vibrations/min achieved high hatching rates (93.58 %) and survival rates (75.67 %). Moreover, bronopol or hydrogen peroxide might have a better choice to replace formaldehyde if further exploration was conducted to reduce stimulation of the in vitro-grown egg. These conditions could be used on a large scale to optimize the production of redclaw crayfish.
