Nature Communications (Mar 2020)

Activation and self-inactivation mechanisms of the cyclic oligoadenylate-dependent CRISPR ribonuclease Csm6

  • Carmela Garcia-Doval,
  • Frank Schwede,
  • Christian Berk,
  • Jakob T. Rostøl,
  • Ole Niewoehner,
  • Oliver Tejero,
  • Jonathan Hall,
  • Luciano A. Marraffini,
  • Martin Jinek

DOI
https://doi.org/10.1038/s41467-020-15334-5
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 9

Abstract

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Upon target RNA recognition, type III CRISPR-Cas systems produce cyclic oligoadenylates that activate effectors such as Csm6 ribonucleases. Here, Garcia-Doval et al. show that Enteroccocus italicus Csm6 degrades its cyclic hexa-AMP activator, and report the crystal structure of the protein bound to an activator mimic.