Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

Yawei Shen; Yudong He; Yanhui Bi; Jianpeng Chen; Ziwei Zhao; Jun Li; Xiaowu Chen

Aquaculture and Fisheries (Nov 2019)

Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

Yawei Shen,
Yudong He,
Yanhui Bi,
Jianpeng Chen,
Ziwei Zhao,
Jun Li,
Xiaowu Chen

Affiliations

Yawei Shen: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China
Yudong He: Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China
Yanhui Bi: Shanghai Engineering Research Center of Aquaculture, Shanghai, 201306, China
Jianpeng Chen: Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China
Ziwei Zhao: Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, China
Jun Li: CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 26000, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 26000, China
Xiaowu Chen: National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. National Demonstration Center for Experimental Fisheries, Science Education, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China.

Journal volume & issue: Vol. 4, no. 6
pp. 247 – 254

Abstract

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The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities. The gill is the main organ involved in fish osmoregulation and in this study, a cDNA library of the L. maculatus gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity. A total raw data of 5.3G base pairs was obtained, followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean reads. After splicing and clustering, 31,976 unigenes were obtained. The average transcript length was 1327 bp and N50 was 2057 bp. In comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E, CD74, keratin, type I cytoskeleton 13, carbonic anhydrase, betaine-homocysteine S-methyltransferase, and aquaporin 3 (AQP3) genes. The L. maculatus aqp3 (Lm-aqp3) gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel domain. Phylogenetic analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus labrax. Quantitative RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues (stomach, liver, brain, spleen and testis). Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater (20 ppt) fish, suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in L. maculatus. Keywords: Lateolabrax maculatus, Gill, Transcriptome, Aquaporin 3

Published in Aquaculture and Fisheries

ISSN: 2468-550X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.keaipublishing.com/en/journals/aquaculture-and-fisheries

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