MANAS: Journal of Engineering (Dec 2017)
Determination of Amino Acid Composition of Cow’s Milk by LiquidChromatography Using Precolumn Derivatization
Abstract
The application of the procedure of pre-column derivatization with diethyl ethoxymethylenemalonate in the determination of the amino acid content of milk by reversedphase chromatography with diode-matrix detection was investigated. The influence of the pH on the response factor was established. The conditions of modification of amino acids during sample preparation procedure were optimized. A complete separation of 18 amino acids was achieved. The most abundant amino acid in cow's milk was glutamic acid, the concentration of which twice that of leucine and lysine, and three times greater than aspartic acid