Nature Communications (Sep 2022)
A high-performance genetically encoded fluorescent indicator for in vivo cAMP imaging
- Liang Wang,
- Chunling Wu,
- Wanling Peng,
- Ziliang Zhou,
- Jianzhi Zeng,
- Xuelin Li,
- Yini Yang,
- Shuguang Yu,
- Ye Zou,
- Mian Huang,
- Chang Liu,
- Yefei Chen,
- Yi Li,
- Panpan Ti,
- Wenfeng Liu,
- Yufeng Gao,
- Wei Zheng,
- Haining Zhong,
- Shangbang Gao,
- Zhonghua Lu,
- Pei-Gen Ren,
- Ho Leung Ng,
- Jie He,
- Shoudeng Chen,
- Min Xu,
- Yulong Li,
- Jun Chu
Affiliations
- Liang Wang
- Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Chunling Wu
- PKU-IDG–McGovern Institute for Brain Research
- Wanling Peng
- Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences
- Ziliang Zhou
- Molecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University
- Jianzhi Zeng
- PKU-IDG–McGovern Institute for Brain Research
- Xuelin Li
- PKU-IDG–McGovern Institute for Brain Research
- Yini Yang
- PKU-IDG–McGovern Institute for Brain Research
- Shuguang Yu
- State Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences
- Ye Zou
- Department of Biochemistry and Molecular Biophysics, Kansas State University
- Mian Huang
- Department of Biochemistry and Molecular Biophysics, Kansas State University
- Chang Liu
- Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Yefei Chen
- Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Yi Li
- Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology
- Panpan Ti
- Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology
- Wenfeng Liu
- Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Yufeng Gao
- Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Wei Zheng
- Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Haining Zhong
- Vollum Institute, Oregon Health and Science University
- Shangbang Gao
- Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology
- Zhonghua Lu
- Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Pei-Gen Ren
- Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- Ho Leung Ng
- Department of Biochemistry and Molecular Biophysics, Kansas State University
- Jie He
- State Key Laboratory of Neuroscience, Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences
- Shoudeng Chen
- Molecular Imaging Center, Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University
- Min Xu
- Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences
- Yulong Li
- PKU-IDG–McGovern Institute for Brain Research
- Jun Chu
- Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-022-32994-7
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
The second messenger cAMP modulates a wide range of important biological processes. Here, the authors developed a fluorescent indicator termed G-Flamp1, which can accurately report levels of cAMP in living cells.
