Journal of Applied Oral Science (Aug 2011)

In vitro adherence of Candida albicans isolated from patients with chronic periodontitis

  • Adriana Gadotti Machado,
  • Edson Yukio Komiyama,
  • Silvana Soléo Ferreira dos Santos,
  • Antonio Olavo Cardoso Jorge,
  • Fernanda Lourenção Brighenti,
  • Cristiane Yumi Koga-Ito

Journal volume & issue
Vol. 19, no. 4
pp. 384 – 387

Abstract

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Adherence is considered an extremely important virulence factor in yeast. OBJECTIVE: The aim of this study was to analyze the adherence to epithelial cells of C. albicans isolated from patients with chronic periodontitis in comparison to healthy patients. MATERIAL AND METHODS: Candida albicans cells isolated from individuals with chronic periodontitis (n=25) and healthy controls (n=25) were included in this study. Suspensions of C. albicans (10(6) cells/mL) and epithelial cells (10(5) cells/mL) were mixed and incubated at 37ºC for 1 h. The number of yeasts adhered to 25 epithelial cells was counted. RESULTS: The number of C. albicans cells adhered to epithelial cells was statistically higher in the chronic periodontitis group than in the control group (Student's t-test, p=0.000). CONCLUSION:The results of the present study suggest a higher Candida adherence of samples isolated from patients with chronic periodontitis.

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