<span style="font-variant: small-caps;">l</span>-Fucose Synthesis Using a Halo- and Thermophilic <span style="font-variant: small-caps;">l</span>-Fucose Isomerase from Polyextremophilic <em>Halothermothrix orenii</em>

Abstract

l-Fucose isomerase (l-FucI)-mediated isomerization is a promising biotechnological approach for synthesizing various rare sugars of industrial significance, including l-fucose. Extremozymes that can retain their functional conformation under extreme conditions, such as high temperature and salinity, offer favorable applications in bioprocesses that accompany harsh conditions. To date, only one thermophilic l-FucI has been characterized for l-fucose synthesis. Here, we report l-FucI from Halothermothrix orenii (HoFucI) which exhibits both halophilic and thermophilic properties. When evaluated under various biochemical conditions, HoFucI exhibited optimal activities at 50–60 °C and pH 7 with 0.5–1 M NaCl in the presence of 1 mM Mn2+ as a cofactor. The results obtained here show a unique feature of HoFucI as a polyextremozyme, which facilitates the biotechnological production of l-fucose using this enzyme.

Keywords

• l-fucose isomerase%22%2C%22default_operator%22%3A%22AND%22%7D%7D%7D"> <span style="font-variant: small-caps;">l</span>-fucose isomerase
• l-fucose%22%2C%22default_operator%22%3A%22AND%22%7D%7D%7D"> <span style="font-variant: small-caps;">l</span>-fucose
• l-fuculose%22%2C%22default_operator%22%3A%22AND%22%7D%7D%7D"> <span style="font-variant: small-caps;">l</span>-fuculose
• extremophile
• halothermophilic bacteria
• <i>Halothermothrix orenii</i>