Nature Communications (Aug 2020)
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
- Dounia Djeghloul,
- Bhavik Patel,
- Holger Kramer,
- Andrew Dimond,
- Chad Whilding,
- Karen Brown,
- Anne-Céline Kohler,
- Amelie Feytout,
- Nicolas Veland,
- James Elliott,
- Tanmay A. M. Bharat,
- Abul K. Tarafder,
- Jan Löwe,
- Bee L. Ng,
- Ya Guo,
- Jacky Guy,
- Miles K. Huseyin,
- Robert J. Klose,
- Matthias Merkenschlager,
- Amanda G. Fisher
Affiliations
- Dounia Djeghloul
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Bhavik Patel
- Flow Cytometry Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Holger Kramer
- Biological Mass Spectrometry and Proteomics Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Andrew Dimond
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Chad Whilding
- Microscopy Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Karen Brown
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Anne-Céline Kohler
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Amelie Feytout
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Nicolas Veland
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- James Elliott
- Flow Cytometry Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Tanmay A. M. Bharat
- Sir William Dunn School of Pathology, University of Oxford
- Abul K. Tarafder
- Sir William Dunn School of Pathology, University of Oxford
- Jan Löwe
- MRC Laboratory of Molecular Biology
- Bee L. Ng
- Wellcome Sanger Institute, Wellcome Genome Campus
- Ya Guo
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Jacky Guy
- The Wellcome Centre for Cell Biology, University of Edinburgh
- Miles K. Huseyin
- Department of Biochemistry, University of Oxford
- Robert J. Klose
- Department of Biochemistry, University of Oxford
- Matthias Merkenschlager
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- Amanda G. Fisher
- Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital Campus
- DOI
- https://doi.org/10.1038/s41467-020-17823-z
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 15
Abstract
Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.
