High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

Abstract

In vitro chemotactic assays are often 2D, low-throughput and lack fine gradient control. Here the authors present a hydrogel-based microfluidic platform for high-throughput 3D chemotactic assays, and use it to study neuronal sensitivity to guidance molecule gradient steepness.