Frontiers in Plant Science (Jun 2022)
The Adaxial/Abaxial Patterning of Auxin and Auxin Gene in Leaf Veins Functions in Leafy Head Formation of Chinese Cabbage
- Xiaozhen Yue,
- Xiaozhen Yue,
- Xiaozhen Yue,
- Xiaozhen Yue,
- Xiaozhen Yue,
- Tongbing Su,
- Tongbing Su,
- Tongbing Su,
- Tongbing Su,
- Xiaoyun Xin,
- Xiaoyun Xin,
- Xiaoyun Xin,
- Xiaoyun Xin,
- Peirong Li,
- Peirong Li,
- Peirong Li,
- Peirong Li,
- Weihong Wang,
- Weihong Wang,
- Weihong Wang,
- Weihong Wang,
- Yangjun Yu,
- Yangjun Yu,
- Yangjun Yu,
- Yangjun Yu,
- Deshuang Zhang,
- Deshuang Zhang,
- Deshuang Zhang,
- Deshuang Zhang,
- Xiuyun Zhao,
- Xiuyun Zhao,
- Xiuyun Zhao,
- Xiuyun Zhao,
- Jiao Wang,
- Liling Sun,
- Guihua Jin,
- Shuancang Yu,
- Shuancang Yu,
- Shuancang Yu,
- Shuancang Yu,
- Fenglan Zhang,
- Fenglan Zhang,
- Fenglan Zhang,
- Fenglan Zhang
Affiliations
- Xiaozhen Yue
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Xiaozhen Yue
- National Engineering Research Center for Vegetables, Beijing, China
- Xiaozhen Yue
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Xiaozhen Yue
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Xiaozhen Yue
- Key Laboratory of the Vegetable Postharvest Treatment of Ministry of Agriculture, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Institute of Agri-Food Processing and Nutrition (IAPN), Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
- Tongbing Su
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Tongbing Su
- National Engineering Research Center for Vegetables, Beijing, China
- Tongbing Su
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Tongbing Su
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Xiaoyun Xin
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Xiaoyun Xin
- National Engineering Research Center for Vegetables, Beijing, China
- Xiaoyun Xin
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Xiaoyun Xin
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Peirong Li
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Peirong Li
- National Engineering Research Center for Vegetables, Beijing, China
- Peirong Li
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Peirong Li
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Weihong Wang
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Weihong Wang
- National Engineering Research Center for Vegetables, Beijing, China
- Weihong Wang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Weihong Wang
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Yangjun Yu
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Yangjun Yu
- National Engineering Research Center for Vegetables, Beijing, China
- Yangjun Yu
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Yangjun Yu
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Deshuang Zhang
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Deshuang Zhang
- National Engineering Research Center for Vegetables, Beijing, China
- Deshuang Zhang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Deshuang Zhang
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Xiuyun Zhao
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Xiuyun Zhao
- National Engineering Research Center for Vegetables, Beijing, China
- Xiuyun Zhao
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Xiuyun Zhao
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Jiao Wang
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Liling Sun
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Guihua Jin
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Shuancang Yu
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Shuancang Yu
- National Engineering Research Center for Vegetables, Beijing, China
- Shuancang Yu
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Shuancang Yu
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- Fenglan Zhang
- Beijing Vegetable Research Center (BVRC), Beijing Academy of Agriculture and Forestry Science (BAAFS), Beijing, China
- Fenglan Zhang
- National Engineering Research Center for Vegetables, Beijing, China
- Fenglan Zhang
- Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing, China
- Fenglan Zhang
- Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China
- DOI
- https://doi.org/10.3389/fpls.2022.918112
- Journal volume & issue
-
Vol. 13
Abstract
Leaf curling is an essential prerequisite for the formation of leafy heads in Chinese cabbage. However, the part or tissue that determines leaf curvature remains largely unclear. In this study, we first introduced the auxin-responsive marker DR5::GUS into the Chinese cabbage genome and visualized its expression during the farming season. We demonstrated that auxin response is adaxially/abaxially distributed in leaf veins. Together with the fact that leaf veins occupy considerable proportions of the Chinese cabbage leaf, we propose that leaf veins play a crucial supporting role as a framework for heading. Then, by combining analyses of QTL mapping and a time-course transcriptome from heading Chinese cabbage and non-heading pak choi during the farming season, we identified the auxin-related gene BrPIN5 as a strong candidate for leafy head formation. PIN5 displays an adaxial/abaxial expression pattern in leaf veins, similar to that of DR5::GUS, revealing an involvement of BrPIN5 in leafy head development. The association of BrPIN5 function with heading was further confirmed by its haplo-specificity to heading individuals in both a natural population and two segregating populations. We thus conclude that the adaxial/abaxial patterning of auxin and auxin genes in leaf veins functions in the formation of the leafy head in Chinese cabbage.
Keywords
