Comparison of p53 Protein Expression between Oral Squamous Cell Carcinoma and Verrucous Carcinoma

Abstract

Introduction: Deletion or mutation of tumor suppressor genes plays a significant role in cancer development. Mutation of the p53 tumor suppressor gene is the most common genetic alteration associated with oral cancer. The aim of this study was to compare the expression of p53 protein in oral squamous cell carcinoma and verrucous carcinoma. Methodology: Immunohistochemical analysis of p53 was done using monoclonal anti- p53, clone BP53-12 antibody (SIGMA, USA); immune-stained cells were counted and intensity of immune-staining in nucleus was noted. Results: Positive immunoreactivity for p53 oncoprotein was seen in 70.4% oral squamous cell carcinoma and 71% cases of verrucous carcinoma. Conclusion: No statistical significance was seen between the number of positive staining cases and staining intensity of oral squamous cell carcinoma and verrucous carcinoma. However, statistical significance was observed on quantitative analysis between oral squamous cell carcinoma and verrucous carcinoma.

Keywords

• Carcinoma cell; oral squamous cell; p53; verrucous carcinoma