Downregulation of Hcn1 protein participates in development of overactive bladder in rats

Abstract

Objective To investigate the expression of RNA and activation and inhibition of related signaling pathways in rats with overactive bladder (DO model), and to explore the molecules and targets involved in the occurrence and development of overactive bladder. Methods Rat DO model was prepared by bladder neck ligation. The urodynamic test was used to evaluate the success of establishment. The regular biological methods were used for RNA extract and quality control was completed by corporation. The data analysis was performed by R platform. Q-PCR and Western blotting were employed to confirm the results of sequencing. Results The result of urodynamic test showed that the DO model was successfully constructed. The RNA-seq sequencing results from 6 samples and the subsequent RT-PCR results showed that the 6 genes in DO bladder tissue were highly expressed and 2 were lowly expressed (P < 0.001). Gene set enrichment analysis (GSEA) showed that the inflammatory response-related pathway was activated in the DO model, and voltage-gated sodium channel activity was inhibited (Enrichment score=-0.565, FDR q value=0.044); Western blot analysis confirmed that hyperpolarization-activated cyclic nucleotide-gated channel 1 (Hcn1) channel protein in DO rats was down-expressed (P < 0.001). Conclusion Low expression of Hcn1 channel protein is involved in the occurrence and development of overactive bladder in the rats.

Keywords

• overactive bladder
• hyperpolarization-activated cyclic nucleotide-gated channel 1
• rats