PLoS ONE (Jan 2024)

Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome.

  • Iasmim Santos Mangabeira-Silva,
  • Paulo Eduardo Toscano Soares,
  • Yago Tomaz Vieira da Silva,
  • Beatriz Helena Dantas Rodrigues de Albuquerque,
  • Maryana Thalyta Ferreira Câmera de Oliveira,
  • Larissa Alves Honorato Ferreira,
  • Maria Fernanda Bezerra de Souza,
  • Danyllo Vieira de Lucena,
  • Jessica Marina Paiva Pereira,
  • Roseli Pimentel Pinheiro E Silva,
  • Daniel Carlos Ferreira Lanza

DOI
https://doi.org/10.1371/journal.pone.0289351
Journal volume & issue
Vol. 19, no. 5
p. e0289351

Abstract

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In this study, an extensive analysis of microsatellite markers (Single Tandem Repeats-STRs) in Penaeus vannamei was conducted at an advanced level. The markers were thoroughly examined, characterized, and specific markers located within coding regions were identified. Out of a total of 306 STRs, 117 were classified as perfect markers based on their single repeat motif. Among these perfect markers, 62 were found to be associated with predicted coding genes (mRNA), which were involved in various functions such as binding, catalytic activity, ATP-dependent activity, transcription, structural and molecular regulation. To validate the accuracy of the findings, a sample of nine markers was subjected to in vitro testing, which confirmed the presence of polymorphisms within the population. These results suggest the existence of different protein isoforms within the population, indicating the potential of these markers for application in both population and phenotype-genotype association studies. This innovative approach opens up new possibilities for investigating the impact of genomic plasticity in populations of P. vannamei.