BMC Pulmonary Medicine (Nov 2021)

HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration

  • Yanyu Li,
  • Guomei Su,
  • Yu Zhong,
  • Zhilin Xiong,
  • Tong Huang,
  • Jingyun Quan,
  • Jiewen Huang,
  • Xiaoxia Wen,
  • Chaole Luo,
  • Weilin Zheng,
  • Jinfeng Chen,
  • Junfen Cheng,
  • Weimin Yao,
  • Tianwen Lai

DOI
https://doi.org/10.1186/s12890-021-01726-w
Journal volume & issue
Vol. 21, no. 1
pp. 1 – 12

Abstract

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Abstract Background We have reported that heparin-binding epidermal growth factor (HB-EGF) is increased in patients with chronic obstructive pulmonary disease (COPD) and associated with collagen deposition, but the mechanisms remain unclear. In the present study, we aimed to investigated the inflammatory cytokines secreted by bronchial epithelial cells following exposure to HB-EGF that promoted proliferation and migration of human lung fibroblast. Methods HB-EGF–induced inflammatory cytokines were assayed in two airway epithelial cells (primary human bronchial epithelial cells [HBECs] and BEAS-2B cells). Moreover, the culture supernatants derived from HB-EGF-treated HBECs and BEAS-2B cells were added to human primary lung fibroblasts. The effect of culture supernatants on proliferation and migration of fibroblasts was assessed. Results IL-8 expression was significantly increased in bronchial epithelial cells treated with HB-EGF, which was at least partially dependent on NF-kB pathways activation. HB-EGF–induced IL-8 was found to further promote lung fibroblasts proliferation and migration, and the effects were attenuated after neutralizing IL-8. Conclusions These findings suggest that HB-EGF may be involved in the pathology of airway fibrosis by induction of IL-8 from airway epithelium, subsequently causing lung fibroblasts proliferation and migration. Thus, inhibition of HBEGF and/or IL-8 production could prevent the development of airway fibrosis by modulating fibroblast activation.

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