OncoTargets and Therapy (Mar 2020)
Long Noncoding RNA NEAT1 Upregulates Survivin and Facilitates Gallbladder Cancer Progression by Sponging microRNA-335
Abstract
Facai Yang,1,* Zhaohui Tang,2,* Anqi Duan,3 Bin Yi,3 Ningjia Shen,3 Zhiyuan Bo,3 Lei Yin,3 Bin Zhu,3 Yinghe Qiu,3 Jingdong Li1 1Department of General Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, People’s Republic of China; 2Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, People’s Republic of China; 3Department of Biliary II, Shanghai Eastern Hepatobiliary Surgery Hospital, Naval Military Medical University, Shanghai 200438, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yinghe QiuDepartment of Biliary II, Shanghai Eastern Hepatobiliary Surgery Hospital, Naval Military Medical University, No. 225 Changhai Road, Yangpu District, Shanghai 200438, People’s Republic of ChinaEmail [email protected] LiDepartment of General Surgery, Affiliated Hospital of North Sichuan Medical College, No. 63, Wenhua Road, Shunqing District, Nanchong 637000, People’s Republic of ChinaEmail [email protected]: Gallbladder cancer (GBC) is the most common cancer of the biliary tract, but molecularly targeted therapies are not available for GBC. Loss of microRNA (miR)-335 expression may be a useful predictor of clinical outcomes and the reversal of its loss of expression may be a useful treatment strategy for GBC. In this study, we investigated whether a long noncoding RNA, nuclear paraspeckle assembly transcript 1 (NEAT1) sponges miR-335 in GBC cells.Materials and Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry were used to determine the expression of miR-335; NEAT1; survivin; and Ki67 in GBC cell lines (GBC-SD and SGC-996) and tissue samples from patients (n = 25). Cell Counting Kit-8, colony-formation, and Transwell migration and invasion assays were performed to measure cell proliferation, migration, and invasion. Bioinformatic analysis and dual-luciferase reporter assays were utilized to analyze correlativity.Results: miR-335 overexpression resulted in inhibition of GBC cell proliferation and invasion. In addition, knockdown of NEAT1 resulted in downregulation of survivin expression. As NEAT1 competitively “sponges” miR-335, NEAT1 knockdown resulted in inhibited GBC cell proliferation and invasion in vitro and GBC tumor growth in vivo. Furthermore, NEAT1 was found to be upregulated in GBC samples, and its expression was inversely correlated with miR-335 levels, but positively correlated with survivin levels.Conclusion: These findings indicate that NEAT1 promotes survivin expression by functioning as a competitive endogenous RNA for miR-335 in GBC cells; thus, we have identified a potential biomarker and target for GBC diagnosis and therapy.Keywords: long noncoding RNA, NEAT1, miR-335-5p, competitive endogenous RNA, survivin, gallbladder cancer
