Supplementation of Lactobacillus reuteri isolated from red jungle fowl along with mannanoligosaccharide improves growth performance, immune response and gut health in broiler chicken

Abstract

A total 360 CARIBRO-Vishal broiler chicks were weighed individually and randomly allocated to nine treatment groups, each having five replicates with eight chicks in each, following complete randomized block design (CRD). The experiment was conducted for 6 weeks duration. The nine treatment groups were control fed basal diet (T1), basal diet+bacitracin methylene disalicylate @ 20 mg/kg feed (T2), basal diet+commercial probiotic @ 0.1 g/kg feed (T3), basal diet + Lab isolated Lactobacillus reuteri (LLR) @ 1×106 CFU/g of fermented feed (T4), basal diet+LLR @ 1×107 CFU/g of fermented feed (T5), basal diet+LLR @ 1×108 CFU/g of fermented feed (T6), basal diet+LLR @ 1×106 CFU/g of fermented+0.1% MOS (T7), basal diet+LLR @ 1×107 CFU/g of fermented+0.1% MOS (T8), and basal diet+LLR @ 1×108 CFU/g of fermented feed+ 0.1% MOS (T9). 20% of daily basal ration for broiler chicken was autoclaved and inoculated with 15% of Lactobacillus isolate broth culture having a viable count of 106, 107, and 108 CFU/ml and fermented at 37°C for 24 h before adding to daily ration afresh and was mixed well. Results of the present study revealed that body weight, body weight gain, immune response both humoral and cell mediated was significantly higher in T9 group. Also the pathogenic bacteria count (Salmonella and E.coli) was significantly lower in the GIT of T9 group as compared to other groups. The significantly higher relative expression of growth related genes, IGF-1 and IGF-1R and immune related gene, IL-6 whereas IL-10 and TLR-4 expression were significantly downregulated in T9 group. So, it can be concluded from the present study that Lactobacillus reuteri isolated from the GIT of the red jungle fowl along with MOS is effective in improving the growth performance, immune response and gut health of commercial CARIBRO-Vishal broiler chicken.

Keywords

• Broiler, Body weight gain, Genes, Immunity, Lactobacillus reuteri, Mannanoligosaccharide (MOS)