Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules

Cédric Castrogiovanni; Alessio V. Inchingolo; Jonathan U. Harrison; Damian Dudka; Onur Sen; Nigel J. Burroughs; Andrew D. McAinsh; Patrick Meraldi

doi:10.1038/s41467-022-32421-x

Nature Communications (Aug 2022)

Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules

Cédric Castrogiovanni,
Alessio V. Inchingolo,
Jonathan U. Harrison,
Damian Dudka,
Onur Sen,
Nigel J. Burroughs,
Andrew D. McAinsh,
Patrick Meraldi

Affiliations

Cédric Castrogiovanni: Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva
Alessio V. Inchingolo: Centre for Mechanochemical Cell Biology, University of Warwick
Jonathan U. Harrison: Centre for Mechanochemical Cell Biology, University of Warwick
Damian Dudka: Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva
Onur Sen: Centre for Mechanochemical Cell Biology, University of Warwick
Nigel J. Burroughs: Centre for Mechanochemical Cell Biology, University of Warwick
Andrew D. McAinsh: Centre for Mechanochemical Cell Biology, University of Warwick
Patrick Meraldi: Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva

DOI: https://doi.org/10.1038/s41467-022-32421-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 16

Abstract

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Microtubules are built from GDP-tubulin lattices with small GTP caps at their plus-ends. Here, the authors reveal that microtubules that attach to kinetochores in mitosis contain, in addition to the GTP-cap and the GDP-lattices, a dynamic micron-sized mixed-nucleotide zone.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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