Frontiers in Oncology (Feb 2021)
CyTOF Analysis Reveals a Distinct Immunosuppressive Microenvironment in IDH Mutant Anaplastic Gliomas
- Weilun Fu,
- Weilun Fu,
- Wenjing Wang,
- Wenjing Wang,
- Hao Li,
- Hao Li,
- Yuming Jiao,
- Yuming Jiao,
- Jiancong Weng,
- Jiancong Weng,
- Ran Huo,
- Ran Huo,
- Zihan Yan,
- Zihan Yan,
- Jie Wang,
- Jie Wang,
- Hongyuan Xu,
- Hongyuan Xu,
- Shuo Wang,
- Shuo Wang,
- Jiangfei Wang,
- Jiangfei Wang,
- Dexi Chen,
- Dexi Chen,
- Yong Cao,
- Yong Cao,
- Jizong Zhao,
- Jizong Zhao
Affiliations
- Weilun Fu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Weilun Fu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Wenjing Wang
- Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China
- Wenjing Wang
- Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, China
- Hao Li
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hao Li
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Yuming Jiao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Yuming Jiao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jiancong Weng
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jiancong Weng
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Ran Huo
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Ran Huo
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Zihan Yan
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Zihan Yan
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jie Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jie Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Hongyuan Xu
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Hongyuan Xu
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Shuo Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Shuo Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jiangfei Wang
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jiangfei Wang
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Dexi Chen
- Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing, China
- Dexi Chen
- Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, China
- Yong Cao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Yong Cao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- Jizong Zhao
- Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China
- Jizong Zhao
- China National Clinical Research Center for Neurological Diseases, Beijing, China
- DOI
- https://doi.org/10.3389/fonc.2020.560211
- Journal volume & issue
-
Vol. 10
Abstract
The immune microenvironment is important for the development, progression, and prognosis of anaplastic glioma (AG). This complex milieu has not been fully elucidated, and a high-dimensional analysis is urgently required. Utilizing mass cytometry (CyTOF), we performed an analysis of immune cells from 5 patients with anaplastic astrocytoma, IDH-mutant (AAmut) and 10 patients with anaplastic oligodendroglioma, IDH-mutant and 1p/19q codeletion (AOD) and their paired peripheral blood mononuclear cells (PBMCs). Based on a panel of 33 biomarkers, we demonstrated the tumor-driven immune changes in the AG immune microenvironment. Our study confirmed that mononuclear phagocytes and T cells are the most abundant immunocytes in the AG immune microenvironment. Glioma-associated microglia/macrophages in both AAmut and AOD samples showed highly immunosuppressive characteristics. Compared to those in the PBMCs, the ratios of immune checkpoint-positive exhausted CD4+ T cells and CD8+ T cells were higher at the AG tumor sites. The AAmut immune milieu exhibits more immunosuppressive characteristics than that in AOD.
Keywords
- anaplastic astrocytoma
- anaplastic oligodendroglioma
- CyTOF/mass cytometry
- immune profiling
- microenvironment
- glioma
