Effect of <i>Streptococcus uberis</i> on Gamma Delta T Cell Phenotype in Bovine Mammary Gland
Petr Slama,
Terezie Zavadilova,
Ales Pavlik,
Pavel Horky,
Sylvie Skalickova,
Jiri Skladanka,
Shubhadeep Roychoudhury,
Simona Baldovska,
Adriana Kolesarova,
Roman Konecny,
Vladimir Tancin,
Monika Zouharova
Affiliations
Petr Slama
Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Terezie Zavadilova
Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Ales Pavlik
Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Pavel Horky
Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Sylvie Skalickova
Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Jiri Skladanka
Department of Animal Nutrition and Forage Production, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Shubhadeep Roychoudhury
Department of Life Science and Bioinformatics, Assam University, Silchar 788 011, India
Simona Baldovska
AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, 94976 Nitra, Slovakia
Adriana Kolesarova
Faculty of Biotechnology and Food Sciences, Institute of Applied Biology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
Roman Konecny
Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentska 1668, 370 05 Ceske Budejovice, Czech Republic
Vladimir Tancin
Faculty of Agrobiology and Food Resources, Institute of Animal Husbandry, Slovak Agriculture University in Nitra, Trieda Andreja Hlinku 2, 94976 Nitra, Slovakia
Monika Zouharova
Infectious Diseases and Preventive Medicine Department, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
In this study, we focused analyzing γδ T cells during bovine mammary gland inflammation induced by Streptococcus uberis. A mammary gland cell suspension was obtained using lavage 24, 48, 72, and 168 h after intramammary-induced infection. The proportion of lymphocytes increased during the entire week in which inflammation was present. The γδ T cells were also elevated during inflammation, reaching their peak at 72 h following induced inflammation. The percentage of apoptotic lymphocytes continually increased, with the highest proportion occurring 168 h after S. uberis infection. The results show that γδ T cells may be involved in the resolution of inflammation in bovine mammary glands, with the apoptosis of those cells potentially playing an important role.
