Cancer Management and Research (Feb 2020)

LncRNA HCP5 Stimulates the Proliferation of Non-Small Cell Lung Cancer Cells by Up-Regulating Survivin Through the Down-Regulation of miR-320

  • Li C,
  • Lei Z,
  • Peng B,
  • Zhu J,
  • Chen L

Journal volume & issue
Vol. Volume 12
pp. 1129 – 1134

Abstract

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Chao Li,1 Zhang Lei,2 Bin Peng,1 Jiang Zhu,1 Li Chen3 1Oncology Department, Second People’s Hospital of Jingmen, Jingmen City, Hubei Province 448000, People’s Republic of China; 2Department of Oncology, The Central Hospital of Wuhan Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province 430061, People’s Republic of China; 3Department of Traditional Chinese Medicine, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Art and Science, Xiangyang City, Hubei Province 441021, People’s Republic of ChinaCorrespondence: Li ChenDepartment of Traditional Chinese Medicine, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Art and Science, Jingzhou Street 136#, Xiangyang City, Hubei Province 441021, People’s Republic of ChinaTel +86 0710-3522791Email [email protected]: We explored the roles of lncRNA HCP5 in non-small cell lung cancer (NSCLC).Methods: Levels of HCP5 were measured by performing qPCR and data were compared between non-tumor and NSCLC tissue samples by performing a paired t-test. Expression levels of miR-320 and survivin mRNA in NSCLC tissues were also measured by performing qPCR. The effects of HCP5, miR-320 and survivin overexpression on the proliferation of H23 cells were analyzed by cell proliferation assay.Results: We found that HCP5 was up-regulated in NSCLC and predicted the poor survival of NSCLC patients. HCP5 was negatively correlated with miR-320 but positively correlated with survivin in NSCLC tissues. In NSCLC cells, HCP5 overexpression led to the up-regulated survivin and down-regulated miR-320. Moreover, miR-320 overexpression failed to affect HCP5 but down-regulated survivin. Cell proliferation assay showed that HCP5 and survivin overexpression led to increased, while miR-320 overexpression led to decreased cell proliferation rate. In addition, miR-320 overexpression reduced the effects of HCP5 overexpression.Conclusion: Therefore, HCP5 may stimulate the proliferation of NSCLC cells by up-regulating survivin through the down-regulation of miR-320.Keywords: HCP5, non-small cell lung cancer, miR-320, survivin

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