Actualidades Biológicas (Dec 2007)
VALORES MORFOMÉTRICOS EN LARVAS DE YAMÚ BRYCON AMAZONICUS (PISCES: CHARACIDAE) OBTENIDAS CON SEMEN FRESCO Y CRIOCONSERVADO MORFOMETRIC VALUES IN YAMÚ BRYCON AMAZONICUS (PISCES: CHARACIDAE) LARVAE OBTAINED FROM FRESH AND CRIOPRESERVED SPERM
Abstract
Con el propósito de evaluar diferencias en el desarrollo corporal, el crecimiento y la sobrevivencia de larvas de yamú (Brycon amazonicus) obtenidas a partir de la fertilización de oocitos con semen fresco (SF) y semen crioconservado (SC), machos y hembras de la especie fueron inducidos hormonalmente con extracto de hipófisis de carpa. El semen obtenido fue evaluado y diluido en una solución de yema de huevo, glucosa y dimetilsulfoxido y congelado en vapores de nitrógeno. Las larvas fueron obtenidas de la seminación en seco de oocitos obtenidos de una misma hembra, con semen fresco o crioconservado y evaluadas desde la eclosión (0 horas) hasta 60 horas posteclosión (HPE). Se determinó la longitud total (LT), longitud notocordal (LN), el volumen del saco vitelino (VSV) y la sobrevivencia desde 35 HPE hasta 60 HPE. Para LT sólo hubo diferencias significativas (p In order to evaluate the differences in the corporal growth, development and survival of yamú (Brycon amazonicus), larvae obtained from eggs fertilized with fresh (FS) and cryopreserved (CS) sperm were used. Males and females of yamú were hormonally stimulated with carp pituitary extract. The sperm obtained was evaluated and diluted in an egg yolk, glucose and dimethylsulfoxide solution and frozen in nitrogen vapours. Eggs from the same female were seminated in dry with fresh and cyopreserved sperm and the larvae obtained were evaluated from the hatching (0 hours) until 60 hours post-hatching (HPH). In each larva, the total length (TL), notocordal length (NL), yolk sac volume (YSV) and larvae survival from 35 to 60 HPH were determined. Significant differences in the TL were observed only at 42 and 54 HPH being the larvae from the CS the longest (6.5 ± 0.04 and 6.8 ± 0.04 mm, respectively). In terms of NL, the higher lengths were observed at 0, 3 and 54 HPH; larvae from FS was longer at 0 HPH (3.3 ± 0.02 mm) and larvae from CS were longer at 3 and 54 HPH (4.1 ± 0.05 and 6.6 ± 0.04 mm, respectively). The highest YSV was observed at 9 HPH in larvae from FS (0.6 ± 0.01 mm³). No significant differences were observed in the survival between treatments. In conclusion, in the experimental condition above described, the corporal growth, development and survival of yamú larvae obtained from FS and CS did not change significantly in terms of LT, NL, and YSV until 60 HPH.
