Identification of candidate genes involved in the sugar metabolism and accumulation during pear fruit post-harvest ripening of ‘Red Clapp’s Favorite’ (Pyrus communis L.) by transcriptome analysis

Abstract

Abstract Background Pear (Pyrus spp.) is a popular fruit that is commercially cultivated in most temperate regions. In fruits, sugar metabolism and accumulation are important factors for fruit organoleptic quality. Post-harvest ripening is a special feature of ‘Red Clapp’s Favorite’. Results In this study, transcriptome sequencing based on the Illumina platform generated 23.8 - 35.8 million unigenes of nine cDNA libraries constructed using RNAs from the ‘Red Clapp’s Favorite’ pear variety with different treatments, in which 2629 new genes were discovered, and 2121 of them were annotated. A total of 2146 DEGs, 3650 DEGs, 1830 DEGs from each comparison were assembled. Moreover, the gene expression patterns of 8 unigenes related to sugar metabolism revealed by qPCR. The main constituents of soluble sugars were fructose and glucose after pear fruit post-harvest ripening, and five unigenes involved in sugar metabolism were discovered. Conclusions Our study not only provides a large-scale assessment of transcriptome resources of ‘Red Clapp’s Favorite’ but also lays the foundation for further research into genes correlated with sugar metabolism.

Keywords

• Pear (Pyrus communis L.)
• Quantitative real-time PCR (qPCR)
• RNA-Seq
• Sugar determination