Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

Kento Ojima; Kazuki Shiraiwa; Kyohei Soga; Tomohiro Doura; Mikiko Takato; Kazuhiro Komatsu; Michisuke Yuzaki; Itaru Hamachi; Shigeki Kiyonaka

doi:10.1038/s41467-021-21082-x

Nature Communications (Feb 2021)

Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

Kento Ojima,
Kazuki Shiraiwa,
Kyohei Soga,
Tomohiro Doura,
Mikiko Takato,
Kazuhiro Komatsu,
Michisuke Yuzaki,
Itaru Hamachi,
Shigeki Kiyonaka

Affiliations

Kento Ojima: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Kazuki Shiraiwa: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Kyohei Soga: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
Tomohiro Doura: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University
Mikiko Takato: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Kazuhiro Komatsu: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Michisuke Yuzaki: Department of Physiology, School of Medicine, Keio University
Itaru Hamachi: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University
Shigeki Kiyonaka: Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University

DOI: https://doi.org/10.1038/s41467-021-21082-x
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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The analysis of AMPA-type glutamate receptor (AMPAR) trafficking is essential for understanding molecular mechanisms of learning and memory, but the analytical tools are currently limited. Here, the authors report a method that combines affinity-based receptor labeling and bioorthogonal click chemistry to quantify AMPAR distribution and trafficking under physiological conditions.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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