The Effect of Sodium Butyrate on Radiosensitivity in Glioblastoma Cell

Abstract

INTRODUCTION: Glioblastoma (GB) is a highly aggressive and malignant brain tumor. Currently there is no cure therapy for GB. Radiotherapy (RT) has an important role in the success of GB treatment. Therefore, the studies regarding with to increase the radiosensitivity of cells by applying of the combine of the molecules having chemotherapeutic potential with RT gain importance. Epigenetic mechanisms play role in both RT and chemotherapy response. The aim of this study was to investigate the effect of sodium butyrate, a histone deacetylase inhibitor, onto the radiosensitivity when combined with RT in GB cells. METHODS: For this purpose, different concentrations of SB (0.5,1,2,3,4,6 and 8 mM) and 8Gy RT were administered to LN-405 cell lines alone and in combination. 3D conformal technique was used in RT application. Cell viability was determined by MTT after incubation 72h. RESULTS: It is detected that SB reduced cell viability on concentration-dependent but RT-independent. Cell viability in the 0.5, 1 and 2 mM groups of SB combination with RT decreased compared to the groups treated with SB alone 31.4%, 36.9% and 17.6%, respectively. According to the results, it is detected that SB increased radiosensitivity at 0.5,1,2,8 mM concentrations. DISCUSSION AND CONCLUSION: This study showed that SB may increase the therapeutic effects of RT applications depending on the concentration. In addition, it is the first study to increase the radiosensitivity of SB by applying 3-dimensional conformal RT procedures in terms of the method of applying RT to the cell series in which acute radiation dose is given in a single fraction.

Keywords

• radiosensitivity
• glioblastoma
• sodium butyrate