Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture
Alima Murtazina,
Gloria Ruiz Alcala,
Yaiza Jimenez-Martinez,
Juan Antonio Marchal,
Anel Tarabayeva,
Elmira Bitanova,
Gordon McDougall,
Nazira Bishimbayeva,
Houria Boulaiz
Affiliations
Alima Murtazina
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
Gloria Ruiz Alcala
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
Yaiza Jimenez-Martinez
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
Juan Antonio Marchal
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
Anel Tarabayeva
Department of General Immunology, Faculty of Medicine, Asfendyarov Kazakh National Medical University, Almaty A35B8H9, Kazakhstan
Elmira Bitanova
Department of General Immunology, Faculty of Medicine, Asfendyarov Kazakh National Medical University, Almaty A35B8H9, Kazakhstan
Gordon McDougall
Plant Biochemistry and Food Quality Group, Environmental and Biochemical Sciences Department, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
Nazira Bishimbayeva
Research Center “Bioscience Technologies”, Almaty A15G7B0, Kazakhstan
Houria Boulaiz
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
There is a global need to discover effective anti-cancerous compounds from natural sources. Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides. In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase and induced different rates of apoptosis (≤48%). Transmission and scanning electron microscopy revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the anti-cancer capacity of WCCPS fractions in colorectal cancer.
