TYPING OF VIBRIO CHOLERAE NON O1/NON O139 STRAINS, ISOLATED IN ROSTOV REGION IN 2014

Abstract

Aim. Comparative study of antibiotics resistance and VNTR-typing of Vibrio cholerae non O1/ non O139 strains, isolated on the territory of Rostov region in 2014. Materials and methods. Antibioticogramms of strains were determined by serial dilution method in dense nutrient medium according to MG 4.2.2495-09 (2009). Pheno-, sero- and VNTR-typing was carried out by conventional methods. Results. The studied strains belonged to V. cholerae species, did not agglutinate with O1 and O139 sera, were atoxigenic, hemolysis-positive, did not contain genes of cholera toxin and toxin-coregulating pili ofadhesion, contained genes ofhemagglutinin/protease, protease PrtV, collagenase, cytotonic factor Cef, outer membrane protein OmpW, tol- and vps-clusters, regulatory genes toxR and hapR. Antibioticogramms of the strains have shown the presence of cultures, resistant to ampicillin, ceftazidime, furazolidone, trimethoprim/sulfamethoxazole with intermediate resistance to streptomycin, kanamycin, gentamycin, amikacin, netilmicin. Approximately 20% of isolates had multiple drug resistance. Data of VNTR- and geno-typing confirmed a possibility of water transmission route of the infection. Conclusion. Execution of monitoring of cultures from environmental samples is necessary for timely detection of genetic characteristics, antibiotics resistance.

Keywords

• vntr-генотип
• non o1/non o139 serogroup cholera vibrios
• antibiotics resistance
• vntr-genotype