Vegetative cell wall protein OsGP1 regulates cell wall mediated soda saline-alkali stress in rice
Fengjin Zhu,
Huihui Cheng,
Jianan Guo,
Shuomeng Bai,
Ziang Liu,
Chunxi Huang,
Jiayi Shen,
Kai Wang,
Chengjun Yang,
Qingjie Guan
Affiliations
Fengjin Zhu
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang Province, China
Huihui Cheng
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang Province, China
Jianan Guo
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang Province, China
Shuomeng Bai
Aulin College, Northeast Forestry University, Harbin, Heilongjiang Province, China
Ziang Liu
College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China
Chunxi Huang
College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China
Jiayi Shen
College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China
Kai Wang
College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China
Chengjun Yang
College of Forestry, Northeast Forestry University, Harbin, Heilongjiang Province, China
Qingjie Guan
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang Province, China
Plant growth and development are inhibited by the high levels of ions and pH due to soda saline-alkali soil, and the cell wall serves as a crucial barrier against external stresses in plant cells. Proteins in the cell wall play important roles in plant cell growth, morphogenesis, pathogen infection and environmental response. In the current study, the full-length coding sequence of the vegetative cell wall protein gene OsGP1 was characterized from Lj11 (Oryza sativa longjing11), it contained 660 bp nucleotides encoding 219 amino acids. Protein-protein interaction network analysis revealed possible interaction between CESA1, TUBB8, and OsJ_01535 proteins, which are related to plant growth and cell wall synthesis. OsGP1 was found to be localized in the cell membrane and cell wall. Furthermore, overexpression of OsGP1 leads to increase in plant height and fresh weight, showing enhanced resistance to saline-alkali stress. The ROS (reactive oxygen species) scavengers were regulated by OsGP1 protein, peroxidase and superoxide dismutase activities were significantly higher, while malondialdehyde was lower in the overexpression line under stress. These results suggest that OsGP1 improves saline-alkali stress tolerance of rice possibly through cell wall-mediated intracellular environmental homeostasis.
