Coffee Ring Effect Enhanced Surface Plasmon Resonance Imaging Biosensor via 2-λ Fitting Detection Method
Youjun Zeng,
Dongyun Kai,
Zhenxiao Niu,
Zhaogang Nie,
Yuye Wang,
Yonghong Shao,
Lin Ma,
Fangteng Zhang,
Guanyu Liu,
Jiajie Chen
Affiliations
Youjun Zeng
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Dongyun Kai
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Zhenxiao Niu
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Zhaogang Nie
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Yuye Wang
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Yonghong Shao
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Lin Ma
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Fangteng Zhang
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Guanyu Liu
School of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, China
Jiajie Chen
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
SPR biosensors have been extensively used for investigating protein–protein interactions. However, in conventional surface plasmon resonance (SPR) biosensors, detection is limited by the Brownian-motion-governed diffusion process of sample molecules in the sensor chip, which makes it challenging to detect biomolecule interactions at ultra-low concentrations. Here, we propose a highly sensitive SPR imaging biosensor which exploits the coffee ring effect (CRE) for in situ enrichment of molecules on the sensing surface. In addition, we designed a wavelength modulation system utilizing two LEDs to reduce the system cost and enhance the detection speed. Furthermore, a detection limit of 213 fM is achieved, which amounts to an approximately 365 times improvement compared to traditional SPR biosensors. With further development, we believe that this SPR imaging system with high sensitivity, less sample consumption, and faster detection speed can be readily applied to ultra-low-concentration molecular detection and interaction analysis.
