Evolutionarily Conserved Roles for Apontic in Induction and Subsequent Decline of Cyclin E Expression
Xian-Feng Wang,
Jin-Xiao Liu,
Zhi-Yuan Ma,
Yang Shen,
Hao-Ran Zhang,
Zi-Zhang Zhou,
Emiko Suzuki,
Qing-Xin Liu,
Susumu Hirose
Affiliations
Xian-Feng Wang
Department of Gene Function and Phenomics, National Institute of Genetics, 1111 Yata, Mishima City, Shizuoka 411-8540, Japan; State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Jin-Xiao Liu
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Zhi-Yuan Ma
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Yang Shen
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Hao-Ran Zhang
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Zi-Zhang Zhou
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China
Emiko Suzuki
Department of Gene Function and Phenomics, National Institute of Genetics, 1111 Yata, Mishima City, Shizuoka 411-8540, Japan
Qing-Xin Liu
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China; Corresponding author
Susumu Hirose
Department of Gene Function and Phenomics, National Institute of Genetics, 1111 Yata, Mishima City, Shizuoka 411-8540, Japan; Corresponding author
Summary: Cyclin E is a key factor for S phase entry, and deregulation of Cyclin E results in developmental defects and tumors. Therefore, proper cycling of Cyclin E is crucial for normal growth. Here we found that transcription factors Apontic (Apt) and E2f1 cooperate to induce cyclin E in Drosophila. Functional binding motifs of Apt and E2f1 are clustered in the first intron of Drosophila cyclin E and directly contribute to the cyclin E transcription. Knockout of apt and e2f1 together abolished Cyclin E expression. Furthermore, Apt up-regulates Retinoblastoma family protein 1 (Rbf1) for proper chromatin compaction, which is known to repress cyclin E. Notably, Apt-dependent up-regulation of Cyclin E and Rbf1 is evolutionarily conserved in mammalian cells. Our findings reveal a unique mechanism underlying the induction and subsequent decline of Cyclin E expression.
