Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture
Alexia Daoust,
Yasmina Saoudi,
Jacques Brocard,
Nora Collomb,
Cécile Batandier,
Mariano Bisbal,
Murielle Salomé,
Annie Andrieux,
Sylvain Bohic,
Emmanuel Barbier
Affiliations
Alexia Daoust
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Yasmina Saoudi
Inserm, Grenoble, France, Université Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Jacques Brocard
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Nora Collomb
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Cécile Batandier
Laboratoire de Bioénergétique Fondamentale et Appliquée, Grenoble, France
Mariano Bisbal
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Murielle Salomé
European Synchrotron Radiation Facility (ESRF), Grenoble, France
Annie Andrieux
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Sylvain Bohic
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France, , European Synchrotron Radiation Facility (ESRF), Grenoble, France
Emmanuel Barbier
Inserm, Grenoble, FranceUniversité Joseph Fourier, Grenoble Institut des Neurosciences, Grenoble, France
Compared to an in vivo experiment, neuronal cell cultures are immediately accessible to observation and manipulation. In this protocol, we describe a technique to evaluate the cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).This protocol is similar to "Neuron-enriched Cultures (Method 2)" protocol (Gao, 2011).
