In Vitro Biofilm Formation by Uropathogenic Bacteria and their Antibiotic Susceptibility Pattern

Abstract

Background: Uropathogens have an ability to form biofilm in urinary tract. Microorganisms growing in biofilm are associated with chronic and recurrent UTI. They are highly resistant to a variety of antimicrobial agents. There are different phenotypic methods to detect biofilm production like Tube Adherence Method (TAM), Congo Red Agar Method (CRAM), Tissue Culture Plate Method (TCPM), etc. Aim and Objectives: The purpose of the study was to observe biofilm formation by uropathogens, their antibiotic resistance pattern and to correlate biofilm formation with drug resistance. Material and Methods: Total 168 isolates were collected from urine over six months. They were subjected to AST by Kirby Bauer disc diffusion method. Detection of biofilm production was done by TAM, CRAM, and TCPM. Results: Escherichia coli was the commonest isolate. Of the 68 clinical isolates, 54% were positive for biofilm production by TAM, 58% by CRAM, and 66% by TCPM. Compared to non-biofilm producers higher antibiotic resistance was observed among biofilm producers. TCPM was found to be more accurate. Conclusion: E. coli was the most frequent isolate. Biofilm producers were found to be resistant for multiple drugs. TCPM was found to be more quantitative and reliable

Keywords

• Recurrent UTI
• biofilm
• TCPM