Low impact to fixed cell processing aiming transmission electron microscopy

Abstract

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.

Keywords

• cell fixation
• transmission electron microscopy
• low impact
• DENV