Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Christopher R. Cromwell; Keewon Sung; Jinho Park; Amanda R. Krysler; Juan Jovel; Seong Keun Kim; Basil P. Hubbard

doi:10.1038/s41467-018-03927-0

Nature Communications (Apr 2018)

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Christopher R. Cromwell,
Keewon Sung,
Jinho Park,
Amanda R. Krysler,
Juan Jovel,
Seong Keun Kim,
Basil P. Hubbard

Affiliations

Christopher R. Cromwell: Department of Pharmacology, University of Alberta
Keewon Sung: Department of Chemistry, Seoul National University
Jinho Park: Department of Chemistry, Seoul National University
Amanda R. Krysler: Department of Pharmacology, University of Alberta
Juan Jovel: The Applied Genomics Core, Office of Research, University of Alberta
Seong Keun Kim: Department of Chemistry, Seoul National University
Basil P. Hubbard: Department of Pharmacology, University of Alberta

DOI: https://doi.org/10.1038/s41467-018-03927-0
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 11

Abstract

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Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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