Nature Communications (Apr 2018)

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

  • Christopher R. Cromwell,
  • Keewon Sung,
  • Jinho Park,
  • Amanda R. Krysler,
  • Juan Jovel,
  • Seong Keun Kim,
  • Basil P. Hubbard

DOI
https://doi.org/10.1038/s41467-018-03927-0
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Minimizing off-target effects is an important concern for therapeutic applications of CRISPR-Cas9. Here, the authors show that incorporating bridged or locked nucleic acids into crRNA improves editing kinetics and reduces off-target cleavage.