A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production
Oscar Cervantes,
Zaira del Rocio Lopez,
Norberto Casillas,
Peter Knauth,
Nayeli Checa,
Francisco Apolinar Cholico,
Rodolfo Hernandez-Gutiérrez,
Luis Hector Quintero,
Jose Avila Paz,
Mario Eduardo Cano
Affiliations
Oscar Cervantes
Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, Mexico
Zaira del Rocio Lopez
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
Norberto Casillas
Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragán 1421, Col. Olímpica, Guadalajara C.P. 44430, Jalisco, Mexico
Peter Knauth
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
Nayeli Checa
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
Francisco Apolinar Cholico
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
Rodolfo Hernandez-Gutiérrez
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Av. Normalistas 800 Colinas de La Normal, Guadalajara C.P. 44270, Jalisco, Mexico
Luis Hector Quintero
Centro Universitario de Ciencias Económico Administrativas, Universidad de Guadalajara, Periférico Norte 799, Col. Los Belenes, Zapopan C.P. 45100, Jalisco, Mexico
Jose Avila Paz
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
Mario Eduardo Cano
Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, Ocotlan C.P. 47810, Jalisco, Mexico
A ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 285 K of blocking temperature were obtained. Additionally, the zeta potential showed a suitable colloidal stability for cancer therapy assays and the magneto-calorimetric trails determined a high power absorption density. In addition, the oxidative capability of the ferrofluid was corroborated by performing the Fenton reaction with methylene blue (MB) dissolved in water, where the ferrofluid was suitable for producing reactive oxygen species (ROS), and surprisingly a strong degradation of MB was also observed when it was combined with H2O2. The intracellular ROS production was qualitatively corroborated using the HT-29 human cell line, by detecting the fluorescent rise induced in 2,7-dichlorofluorescein diacetate. In other experiments, cell metabolic activity was measured, and no toxicity was observed, even with concentrations of up to 4 mg/mL of magnetic nanoparticles (MNPs). When the cells were treated with magnetic hyperthermia, 80% of cells were dead at 43 °C using 3 mg/mL of MNPs and applying a magnetic field of 530 kHz with 20 kA/m amplitude.