Revista do Instituto de Medicina Tropical de São Paulo (Feb 2019)

Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients

  • Lisandra Serra Damasceno,
  • Tania Vite-Garín,
  • José Antonio Ramírez,
  • Gabriela Rodríguez-Arellanes,
  • Marcos Abreu de Almeida,
  • Mauro de Medeiros Muniz,
  • Jacó Ricarte Lima de Mesquita,
  • Terezinha do Menino Jesus Silva Leitão,
  • Maria Lucia Taylor,
  • Rosely Maria Zancopé-Oliveira

DOI
https://doi.org/10.1590/s1678-9946201961008
Journal volume & issue
Vol. 61, no. 0

Abstract

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ABSTRACT Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.

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