Acta Medica Leopoliensia (Oct 2024)
MORPHOLOGICAL AND RADIOLOGICAL ASPECTS OF POST-TRAUMATIC REMODELING OF THE MANDIBULAR BONE AFTER TRANSPLANTATION OF NATURAL COLLAGEN WITH LINCOMYCIN
Abstract
This article presents the research results of the morphological, radiological, and lectin-histochemical characteristics of bone-ceramic regenerate after the transplantation of natural collagen combined with lincomycin into an experimental defect in the rabbit mandible. Aim. To study in an experiment the dynamics of changes in the mandible bone after its traumatic injury with subsequent replacement of the defect with an osteoplastic material based on natural collagen in combination with lincomycin. Materials and Methods. Experiments were conducted on 45 male rabbits aged 6-7 months, weighing 2.5-3.0 kg. 20 animals constituted the control group, and 20 the experimental group. Another 5 intact animals were used to study the normal structure of the bone tissue of the studied area of the mandible. The control group included animals with a bone tissue defect that healed under a blood clot. The experimental group consisted of rabbits where the bone defect was filled with natural collagen, with simultaneous intramuscular injection of Lincomycin at a dose of 12 mg/kg of animal weight once daily for 6 days (Col-C-L). Post-traumatic bone tissue status within the defect area was monitored for 84 days. The following methods were employed: bone defect modeling, assessment of jaw macrostructure, radiographic examination, radiovisiography, examination of bone sections under a microscope, and lectin-histochemical analysis of decalcified bone sections. Results and Discussion. Examination of the macrostructure of the experimental bone defect in the rabbit mandible after implantation of natural collagen in combination with lincomycin revealed numerous regenerative changes that occurred after the injury and correlated with the data of radiographic and radiovisiographic examinations. Microscopic examination of bone sections allowed us to establish the phased nature of the dynamics of the studied regenerative changes and the composition of the regenerate. Conclusions. It was established that in the experimental group of animals where defect repair was performed using Col-C osteoplastic material with simultaneous prophylactic administration of Lincomycin in the early stages of the experiment, it positively affected the course of the inflammatory process, followed by the formation of new bone tissue.
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