Nature Communications (Mar 2020)

Single-molecule live-cell imaging visualizes parallel pathways of prokaryotic nucleotide excision repair

  • Harshad Ghodke,
  • Han Ngoc Ho,
  • Antoine M. van Oijen

DOI
https://doi.org/10.1038/s41467-020-15179-y
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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In Escherichia coli, the UvrAB damage sensor recognizes helix-distorting lesions by itself or via Mfd bound to stalled RNA polymerase. Here authors use single-molecule fluorescence imaging to quantify the kinetic signatures of interactions of UvrA with Mfd and UvrB in live cells.