Growth factor analysis and desulfurization performance of sulfate-reducing bacteria

Abstract

Sulfate-reducing bacteria （SRB） was a class of bacteria that use organic matter to reduce SO42- to produce sulfides. SRB can be used to treat wastewater containing SO42-，but the desulfurization effect is limited by temperature，environmental pH，S2- concentration，m（COD）/m（SO42-） and other factors. Based on this，this study used batch experiments to comprehensively analyze the effects of temperature，environmental pH，S2- mass concentration and m（COD）/m（SO42-） on the growth of SRB，and explored the desulfurization performance of SRB in different environments. The results showed that SRB was in the logarithmic phase when cultured for 14-86 h，and the activity was the highest. The optimum growth temperature of SRB was 35 ℃. The S2- in the system would affect the growth of SRB. When the mass concentration of S2- increased，it would not only inhibit the metabolic activity of SRB，but also lead to apoptosis of SRB cells. SRB could survive under the condition of pH 5-8. When the pH of the environment was 7-8，the metabolism of SRB was the most vigorous. The optimum m（COD）/m（SO42-） for SRB growth was 2. Under the optimal conditions of temperature 35 ℃，pH=7，and m（COD）/m（SO42-）=2，SRB in logarithmic phase was cultured on Starkey medium for 8 days，the OD600，pH，ORP，EC，and SO42- removal rates of the solution were 1.33，8.78，-393 mV，2.90 mS/cm，and 84.77%，respectively.

Keywords

• sulfate reducing bacteria
• acid mine drainage
• growth factors
• desulfurization performance