Nature Communications (Sep 2019)
Modular enzyme assembly for enhanced cascade biocatalysis and metabolic flux
- Wei Kang,
- Tian Ma,
- Min Liu,
- Jiale Qu,
- Zhenjun Liu,
- Huawei Zhang,
- Bin Shi,
- Shuai Fu,
- Juncai Ma,
- Louis Tung Faat Lai,
- Sicong He,
- Jianan Qu,
- Shannon Wing-Ngor Au,
- Byung Ho Kang,
- Wilson Chun Yu Lau,
- Zixin Deng,
- Jiang Xia,
- Tiangang Liu
Affiliations
- Wei Kang
- Department of Chemistry, The Chinese University of Hong Kong
- Tian Ma
- Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University
- Min Liu
- Department of Chemistry, The Chinese University of Hong Kong
- Jiale Qu
- Department of Chemistry, The Chinese University of Hong Kong
- Zhenjun Liu
- Department of Chemistry, The Chinese University of Hong Kong
- Huawei Zhang
- School of Life Sciences, The Chinese University of Hong Kong
- Bin Shi
- Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University
- Shuai Fu
- J1 Biotech Co., Ltd.
- Juncai Ma
- School of Life Sciences, The Chinese University of Hong Kong
- Louis Tung Faat Lai
- School of Life Sciences, The Chinese University of Hong Kong
- Sicong He
- Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology
- Jianan Qu
- Department of Electronic and Computer Engineering, Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology
- Shannon Wing-Ngor Au
- School of Life Sciences, The Chinese University of Hong Kong
- Byung Ho Kang
- School of Life Sciences, The Chinese University of Hong Kong
- Wilson Chun Yu Lau
- School of Life Sciences, The Chinese University of Hong Kong
- Zixin Deng
- Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University
- Jiang Xia
- Department of Chemistry, The Chinese University of Hong Kong
- Tiangang Liu
- Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education and School of Pharmaceutical Sciences, Wuhan University
- DOI
- https://doi.org/10.1038/s41467-019-12247-w
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
Metabolic enzymes often form supramolecular complexes to improve product yield. Here the authors use short peptide tags to create scaffold-free assemblies and synthetic metabolic nodes.
