Spleen histology in the female Ohrid trout, Salmo letnica (Kar.) (Teleostei, Salmonidae) during the reproductive cycle

Abstract

The Ohrid trout is an endemic autochthonous species dating from the Tertiary period with an unique ecological and commercial value. We still lack the basic knowledge of normal histology of many visceral organs in this species, including the spleen. To tackle this limitation, the investigation was focused on a histological description of the spleen and the influence of the breeding cycle on some aspects of the spleen (the relative volumes of the red and white pulp). Examination by light microscopy showed the same basic structural features of the salmonid spleen: red and white pulp with randomly distributed melanomacrophage centers, surrounded by a fibrous capsule. White pulp comprises about 35-40% of the volume of the parenchyma, 2-fold less than red pulp. During the breeding cycle, we observed a significant increase of white pulp and decrease of red pulp in the spawning stage compared to earlier stages. After spawning, the relative volumes of white and red pulp exhibit a tendency to decrease and increase, respectively. Based on correlations between the amount of white and red pulp and the ovary somatic ratio, we assumed there could be a connection between the sex steroid status and the pulp content in the spleen.

Keywords

• Ohrid trout
• Salmo letnica
• spleen
• white and red pulp
• stereology